HAV IgM Elisa Test
INTENDED USE
ELISA Kit for IgM Antibody to Hepatitis A Virus is an in vitro enzyme
immunoassay for the detection of HAV-IgM in human serum or plasma.
PRINCIPLE
This kit uses capture ELISA method to detect anti-HAV IgM. The purified
mouse anti-human IgM (μchain) antibody is coated on the solid phase
of multi-wells. A conjugate HAV-Ag is added to the coated wells after
diluted sample added and incubated. Then horseradish peroxidase
labeled HAV-Ag is added. If HAV-IgM is present in the sample, a
complex of Anti-μ-chain-HAV-IgM –HAV-Ag -labeled with HRP will form.
Wash wells to remove other unbounded serum components, incubate
with substrate (TMB) to form a colored product, and measure the
absorbance at 450nm to indicate the presence or absence of HAV-IgM
in the sample. The test is special, sensitive, reproducible and easy to
operate.
| Product details |
Description |
| Delivery |
Within 48 hours |
| Packaging Specifications |
8 x 12 strips, 96 wells |
| Country Of Origin |
China |
| Manufacturer |
18 months |
| Preservation method |
2℃-8℃ |
| Specimen |
Whole blood |
| Assification |
class1 |
| Type |
HAV IgM Elisa Test Kit |
SAMPLE COLLECTION AND PRESERVATION
Blood serum samples are routinely prepared form vein. Blood plasma
samples are routinely prepared with routine amount of anticoagulant
such as heparin or sodium citrate. Sample can be stored at 4°C if tested
within five days. Sample can be stored at -20°C at least for 3 months.
Avoid hemolysis and repetitive freeze and thaw of samples. Samples
with cloud or precipitation should be centrifugated or filtered before test.
Prevent serum from bacterium contamination during collectiing and
storing
TEST PROCEDURE
1. Bring ELISA Kit for Antibody IgM to Hepatitis A Virus (all reagents),
and samples to room temperature before use (approximately 30
minutes).
2. Dilute concentrated wash buffer 1:19 with ddH
3. Dilute the sample (1:1000) with physiological saline.
4. For each test, set one blank, two positive and three negative controls.
Add 100μl positive and negative control serum into positive and
negative control wells respectively.
5. Add 100μl diluted sample into other test wells.
6. Cover wells with seal paper, then incubate 30 minutes at 37°C.
7. Discard the liquid in all wells and fill the wells with wash solution. Lay
aside for 15 seconds, discard the liquid in all wells and fill the wells
with wash solution. Repeat 5 times and dry wells after last wash.
8. Add 50 μl conjugate HAV-Ag in each well except the blank.
9. Addμl 50 μl Enzyme Conjugant in each well except the blank
10.Cover wells with seal paper, then incubate 30 minutes at 37°C.
11. Repeat step 7.
12.Add 50 μl substrate A and B respectively to each well,mix gently
protected from light and incubate 15 minutes at 37°C.
13. Add 50μl of stop solution into each well to stop the reaction,
including blank well.
14. Measure the absorbance at 450 nm against the blank, or measure
the absorbance at 450 nm/630-690 nm.
