Nipah virus Real Time RT-PCR Kit
INTENDED USE
The Nipah virus Real Time RT-PCR Kit is an in vitro nucleic acid
amplification test for qualitative detection of Nipah virus RNA in the
biological material (swab, blood plasma, serum, cerebrospinal fluid)
using real-time hybridization-fluorescence detection of amplified
products.
| Product details |
Description |
| Delivery |
Within 48 hours |
| Packaging Specifications |
8 x 12 strips, 96 wells |
| Country Of Origin |
China |
| Manufacturer |
18 months |
| Preservation method |
-20℃±5℃
|
| Specimen |
swab, blood plasma, serum, cerebrospinal fluid |
| Assification |
class1 |
| Type |
pcr |
SUMMARY
Nipah virus (NiV) is a negative-sense, single-stranded,
non-segmented RNA virus,in which the genome consists of six genes
encoding for the nucleocapsid(N), phosphoprotein (P), matrix protein
(M), fusion protein (F), glycoprotein (G), and the RNA-dependent
RNA polymerase protein (L).
Nipah virus (NiV) is a zoonotic pathogen with airborne transmission
and high case fatality rates in humans.There is currently no treatment or
vaccine against NiV infection approved for humans or animals,
therefore early diagnosis is the key to control any potential outbreaks.
Two presentative viral strains of NiV, namely NiV-B and NiV-M,
were identified separately from some isolates in Bangladesh, India, and
Malaysia. Although they share 91.8% homology similarities, the NiV-B
strain is verified to have a higher fatality rate with ranging from 40 to
75%. In the early stage of human infection, non-specific symptoms
such as fever, headache, and myalgia appear. As the disease progresses,
it
can cause
encephalitis, with symptoms like disorientation,
convulsions, and coma. Severe cases are accompanied by acute
respiratory distress syndrome. The incubation period is usually 4-14
days
PRINCIPLE
Nipah virus detection by the polymerase chain reaction (PCR) is
based on the RNA extraction form the test material and subsequent
simultaneous
carrying out of reverse transcription reaction and
amplification of Nipah virus virus cDNA fragments and Internal
Control (IC) DNA with hybridization-fluorescence detection.
The RNA obtained at the RNA extraction stage is reverse transcribed
into DNA by using the Revertase enzyme, and then amplified into
cDNA fragments by using specific primers and the enzyme Taq
polymerase. In the real-time PCR, the amplified product is detected
with
the
use of
fluorescent dyes. These dyes are linked to
oligonucleotide probes, which bind specifically to the amplified
product
during
thermocycling.
The real-time monitoring of
fluorescence intensities during the real-time PCR allows the detection
of accumulating product without re-opening the reaction tubes after the
PCR run.
At the RT-PCR stage, 2 reactions are carried out simultaneously in
one tube – amplification of the Nipah virus cDNA and the Internal
Control (IC) DNA sequences. The amplification results of Nipah virus
cDNA and Internal Control (IC) DNA are detected in 2 different
fluorescence detection channels:
the Nipah virus cDNA is detected in the FAM channel, the IC DNA
is detected in the ROX channel.
Storage and validity
1．Storage: 2-8℃.
2．validity: six months
Japanese
